Phenotypical and genotypical analysis of the Trueperella bernardiae strain isolated from a dog

Authors

  • Wyanda Arnafia
  • Siti Gusti Ningrum
  • Osama Sammra
  • Mazen Alssahen
  • Jörn-Peter Wickhorst
  • Christoph Lämmler
  • Ellen Prenger-Berninghoff
  • Markus Timke
  • Amir Abdulmawjood

Keywords:

Trueperella bernardiae, dog, MALDI-TOF, 16S rDNA, rpoB, gap

Abstract

The present study was designed to characterize phenotypically and genotypically the Trueperella bernardiae strain (T. bernardiae P5459/15) isolated from the purulent thelitis of a dog. The species identity could be confirmed by phenotypical investigations, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, and sequencing various molecular targets. Sequencing the 16S rDNA, the β subunit of bacterial RNA polymerase encoding gene rpoBand, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap revealed sequence similarities to the type strain T. bernardiae DSM 9152 of 99.6%, 99.3% and 99.1%, respectively. The present study is the first phenotypic and genotypic characterization of the T. bernardiae strain isolated from a dog.

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Published

2020-12-25

Issue

Section

Research Article (peer review)