Phenotypic and genotypic analysis of an arcanobacterium pluranimalium isolated from a muskox (Ovibos moschatus)
Keywords:Arcanobacterium pluranimalium, muskox, MALDI-TOF MS, 16S rDNA, rpoB
The present study was designed to characterize an Arcanobacterium pluranimalium strain isolated from a muskox (Ovibos moschatus) phenotypically, by MALDI-TOF MS analysis and genotypically using various molecular targets. The phenotypic properties, the MALDI-TOF MS analysis and sequencing the 16S rRNA gene, the b subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf and the pluranimaliumlysin encoding gene pla allowed a successful identification of the isolated strain as A. pluranimalium. Gene pla could also be detected by a previously described loop-mediated isothermal amplification (LAMP) assay. This is first report on the isolation and characterization of A. pluranimalium originated from a Muskox.
Copyright (c) 2020 Siti Gusti Ningrum, Wyanda Arnafia, Osama Sammra, Mazen Alssahen, Jorn-Peter Wickhorst, Abdulwahed Ahmed Hassan, Christoph Lammler , Ellen Prenger-Berninghoff, Markus Timke, Amir Abdulmawjood
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