Phenotypic and genotypic analysis of an arcanobacterium pluranimalium isolated from a muskox (Ovibos moschatus)

Authors

  • Siti Gusti Ningrum
  • Wyanda Arnafia
  • Osama Sammra
  • Mazen Alssahen
  • Jorn-Peter Wickhorst
  • Abdulwahed Ahmed Hassan
  • Christoph Lammler
  • Ellen Prenger-Berninghoff
  • Markus Timke
  • Amir Abdulmawjood

Keywords:

Arcanobacterium pluranimalium, muskox, MALDI-TOF MS, 16S rDNA, rpoB

Abstract

The present study was designed to characterize an Arcanobacterium pluranimalium strain isolated from a muskox (Ovibos moschatus) phenotypically, by MALDI-TOF MS analysis and genotypically using various molecular targets. The phenotypic properties, the MALDI-TOF MS analysis and sequencing the 16S rRNA gene, the b subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf and the pluranimaliumlysin encoding gene pla allowed a successful identification of the isolated strain as A. pluranimalium. Gene pla could also be detected by a previously described loop-mediated isothermal amplification (LAMP) assay. This is first report on the isolation and characterization of A. pluranimalium originated from a Muskox.

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Published

2020-12-25

Issue

Section

Research Article (peer review)