Detection of G1 point mutation (c.2039G>A) in GDF9 gene of MEGA (Merino × Garut) sheep

Authors

  • Widya Pintaka Bayu Putra Putra Reseach Center for Applied Zoology - National Research and Innovation Agency
  • Endang Tri Margawati Research Center for Applied Zoology - National Research and Innovation Agency, Indonesia
  • Selintaria Pardede Department of Animal Science - Faculty of Agriculture, Bengkulu University, Bengkulu, Indonesia
  • Edi Soetrisno Department of Animal Science - Faculty of Agriculture, Bengkulu University, Bengkulu, Indonesia
  • Herman Willem Raadsma Center for Advanced Technologies of in Animal Genetics and Reproduction - Faculty of Veterinary, University of Sydney, Camden, NSW, Australia

DOI:

https://doi.org/10.51607/22331360.2022.71.3.357

Keywords:

Exon 1, G1 point mutation, GDF9 gene, MEGA sheep, sequencing

Abstract

The G1 point mutation (R87H) has been used as molecular selection for prolificacy trait in sheep. This mutation occured at c.2039G>A (exon 1) of GDF9 gene (GenBank: AF078545.2) and produced a desirable FecG1 allele. This study was aimed to detect the evidence of G1 point mutation in MEGA (Merino × Garut) sheep with a sequencing method. Detection of G1 point mutation in this study was conducted by applying thirty (30) MEGA sheep at the breeding station. Research showed that a carrier FecG1 (A) allele was detected only in one animal sample. Hence, the frequency of FecG1 allele in MEGA sheep was very low (0.02). It can be concluded that the presence of FecG1 allele in MEGA sheep was rarely detected. In the future, the dept study with large animal sample and another regions of GDF9 gene observation are important to obtain the genetic marker for reproductive traits of MEGA sheep.

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Published

2022-12-18

Issue

Section

Short Communications (peer review)